基因组测序文件：菌株SYSU D00978T 的16S rRNA 基因正反序列文件。\n基因组组装文件：使用 Fastp（v0.23.2）对原始读取进行修剪以去除低质量碱基，并使用工具 SPAdes （v3.15）组装产生的高质量序列。
", "ds_source": "在2021 年中国西北旱地微生物多样性研究中，从新疆古尔班通古特沙漠（北纬44°56′，东经88°33′，海拔 654 m）采集的沙质块状土壤中分离出来菌株SYSU D00978T。
", "ds_process_way": "将大约 5 g 原始土壤悬浮在 45 mL 无菌 0.85% 生理盐水中，并在 28 °C 和 180 rpm 下摇动 60 分钟。制备连续 10 倍稀释液，并将 100 μL 等分试样涂布到淀粉酪蛋白琼脂上（SCA：可溶性淀粉 10.0 g/L，酪蛋白 0.3 g/L，KNO3 2.0 g/L，NaCl 2.0 g/L，K2HPO4 2.0 g/L，MgSO4·7H2O 0.05 g/L，CaCO3 0.02 g/L，FeSO4·7H2O 0.01 g/L，琼脂 18.0 g/L，pH 7.2），含有选择性试剂（制霉菌素 50 mg/L、萘啶酸 25 mg/L、放线菌酮 25 mg/L 和重铬酸钾 25 mg/L）抑制真菌和快速生长的细菌。培养基补充了基于国际链霉菌项目 （ISP）的微量盐和维生素，包括额外的钴胺素（0.5 mg/L）和叶酸（0.5 mg/L）。\n将板在 30 ℃下孵育5天，然后通过在改良的 ISP 4 琼脂（可溶性淀粉 10.0 g/L，酵母提取物 4 g/L，（NH4)2SO4 2.0 g/L，CaCO3 2.0 g/L，K2HPO4 1.0 g/L，MgSO4·7H2O 1.0 g/L，NaCl 1.0 g/L，琼脂 18.0 g，pH 7.2）上重复划线来选择和纯化黄色菌落（SYSU D00978T）。纯化的菌株在-80 °C 下保存为甘油原液 （25 %，v/v），在 4 °C 下保存为冻干奶管 （10 %，m/v）。\n使用 E.Z.N.A.® 细菌 DNA 试剂盒从纯化培养生物质中提取菌株 SYSU D00978T 的基因组 DNA，使用16S rRNA基因进行扩增和测序，使用ContEst16S 算法从草图基因组中提取完整的16S rRNA基因序列（1521 bp）。\n在北京基因组研究所，使用 Illumina NovaSeq 6000 平台对菌株 SYSU D00978T 进行了全基因组测序，使用 Fastp（v0.23.2）对原始读取进行修剪以去除低质量碱基，并使用工具 SPAdes （v3.15）组装产生的高质量序列。\n基因组原始测序数据及高质量基因组数据已上传至NCBI数据库，项目号为PRJNA699703，基因组登录号为JAFIQW000000000.1。
", "ds_quality": "", "ds_acq_start_time": "2021-03-25 00:00:00", "ds_acq_end_time": "2021-03-25 00:00:00", "ds_acq_place": "新疆古尔班通古特沙漠（北纬44°56′，东经88°33′，海拔 654 m）", "ds_acq_lon_east": null, "ds_acq_lat_south": null, "ds_acq_lon_west": null, "ds_acq_lat_north": null, "ds_acq_alt_low": null, "ds_acq_alt_high": null, "ds_share_type": "open-access", "ds_total_size": 725200741, "ds_files_count": 3, "ds_format": "gz和fasta文件", "ds_space_res": null, "ds_time_res": "", "ds_coordinate": "无", "ds_projection": "无", "ds_thumbnail": "89b3ce23-e921-41d0-a765-d544f2371558.png", "ds_thumb_from": 0, "ds_ref_way": "", "paper_ref_way": "", "ds_ref_instruction": "", "ds_from_station": null, "organization_id": "a5877b42-96ea-4f13-af7e-246f355413d6", "doi_value": "", "subject_codes": [ "180.61" ], "quality_level": 1, "publish_time": "2026-06-22 12:59:37", "first_publish_time": null, "last_updated": "2026-06-22 12:59:37", "protected": false, "protected_to": null, "lang": "zh", "cstr": "33110.11.ariddc.01447", "license": null, "extra": null, "files_shape": [ { "name": "基因组测序文件", "size": null, "is_dir": true }, { "name": "基因组组装文件", "size": null, "is_dir": true } ], "features": null, "data_level": 0, "i18n": { "en": { "title": "Genome sequencing file of SYSU D00978T in sandy soil mass in Gurbantongut, Xinjiang", "ds_abstract": "Genome sequencing file: Reverse and reverse sequence file of the 16S rRNA gene of strain SYSU D00978T.\nGenome Assembly File: Raw reads were trimmed using Fastp (v0.23.2) to remove low-quality bases, and the tool SPAdes (v3.15) was used to assemble the resulting high-quality sequence.
", "ds_source": "In the 2021 microbial diversity study on dryland microbial diversity in northwest China, strain SYSU D00978T was isolated from sandy mass soil collected in the Gurbantongut Desert in Xinjiang (44°56 'north latitude, 88°33' east longitude, 654 m above sea level).
", "ds_process_way": "Approximately 5 g of raw soil was suspended in 45 mL of sterile 0.85% physiological saline and shaken at 28 °C and 180 rpm for 60 minutes. Prepare serial 10-fold dilutions and spread 100 μL aliquots onto starch casein agar （SCA： Soluble starch 10.0 g/L, casein 0.3 g/L, KNO3 2.0 g/L, NaCl 2.0 g/L, K2HPO4 2.0 g/L, MgSO4·7H2O 0.05 g/L, CaCO3 0.02 g/L, FeSO4·7H2O 0.01 g/L, agar 18.0 g/L, pH 7.2), Contains selective reagents (nystatin 50 mg/L, nalidixic acid 25 mg/L, actinidone 25 mg/L and potassium dichromate 25 mg/L) to inhibit fungi and fast-growing bacteria. The medium was supplemented with trace salts and vitamins based on the International Streptomyces Project (ISP), including additional cobalamin (0.5 mg/L) and folic acid (0.5 mg/L).\nThe plates were incubated at 30 ℃ for 5 days, and then yellow colonies (SYSU D00978T) were selected and purified by repeating streaking on modified ISP4 agar (soluble starch 10.0 g/L, yeast extract 4 g/L,(NH4) 2SO4 2.0 g/L, CaCO3 2.0 g/L, K2HPO4 1.0 g/L, MgSO4·7H2O 1.0 g/L, NaCl 18.0 g, pH 7.2). The purified strain was stored as a glycerol stock solution (25%, v/v) at-80 °C and as lyophilized milk tubes (10%, m/v) at 4 °C.\nThe genomic DNA of strain SYSU D00978T was extracted from purified cultured biomass using the E.Z.N.A. ® Bacterial DNA Kit, the 16S rRNA gene was amplified and sequenced, and the complete 16S rRNA gene sequence (1521 bp) was extracted from the draft genome using the ContEst16S algorithm.\nAt the Beijing Genome Research Institute, the entire genome of strain SYSU D00978T was sequenced using the Illumina NovaSeq 6000 platform, the original reads were trimmed using Fastp (v0.23.2) to remove low-quality bases, and the resulting high-quality sequence was assembled using the tool SPAdes (v3.15).\nThe original genomic sequencing data and high-quality genomic data have been uploaded to the NCBI database, the project number is PRJNA699703, and the genome accession number is JAFIQW000000000.1.
", "ds_acq_place": "Gurbantungut Desert, Xinjiang (44°56 'N, 88°33' E, 654 m above sea level)", "ds_format": "Gz and fasta files", "ds_projection": "no" } }, "license_type": null, "doi_reg_from": "reg_local", "cstr_reg_from": "reg_local", "doi_not_reg_reason": null, "cstr_not_reg_reason": null, "is_paper_in_submitting": false, "ds_topic_tags": [ "土壤", "菌株", "基因" ], "ds_subject_tags": [ "微生物学" ], "ds_class_tags": [], "ds_locus_tags": [ "新疆古尔班通古特沙漠（北纬44°56′，东经88°33′，海拔 654 m）" ], "ds_time_tags": [ 2021 ], "ds_contributors": [ "李帅" ], "ds_meta_authors": [ "李帅" ], "ds_managers": [ "李帅" ], "category": "土壤" }