伊犁地区植物DNA条形码序列，包括matK, rbcL和ITS基因序列和峰图。306个植物样本进行测序分析，这些样本分属于65科，204属，274个种。其中包括野生林果植物，野生花卉，药用植物和作物近缘种，珍稀，濒危植物等。MatK, rbcL和ITS测序表明，共获得281个rbcL片段, 281个matK片段和278个ITS片段，每个测序中包含上游引物（_f）和下游引物（_r）
", "ds_source": "测序原始数据，未经加工处理。
", "ds_process_way": "野外采集植物样本，进行DNA提取，PCR扩增，碱基测序获得。通过测序仪对植物DNA碱基测序，根据峰图判断碱基读取的准确性，去除序列2端不确定的序列，获得可信度高的碱基序列。fasta测序结果可以用文本打开； 峰图文件（AB1格式）可以使用Chromas软件查看。
", "ds_quality": "关于天山野果林关键物种DNA条形码数据资源遗传材料采集过程依照《县域陆生高等植物多样性调查与评估技术规定》进行人工采集，DNA条形码数据采集参考和引用《关于植物 DNA 条形码研究技术规范》（高连明等，2012）及本次科考生物多样性调查规范相关内容。经过人工检查本年度数据误差值小于0.1%，符合本次科考生物多样性调查规范相关要求。
", "ds_acq_start_time": "2023-01-01 00:00:00", "ds_acq_end_time": "2023-12-31 00:00:00", "ds_acq_place": "伊犁地区", "ds_acq_lon_east": null, "ds_acq_lat_south": null, "ds_acq_lon_west": null, "ds_acq_lat_north": null, "ds_acq_alt_low": null, "ds_acq_alt_high": null, "ds_share_type": "login-access", "ds_total_size": 0, "ds_files_count": 0, "ds_format": "表格", "ds_space_res": null, "ds_time_res": "", "ds_coordinate": "无", "ds_projection": "无", "ds_thumbnail": "19926dca-d215-43b1-82e8-a6d8742df259.jpg", "ds_thumb_from": 2, "ds_ref_way": "", "paper_ref_way": "", "ds_ref_instruction": "天山野果林关键物种DNA条形码数据库（2023年）来源于第三次新疆综合科学考察专项 \"天山野果林生态系统与生物多样性调查(2021xjkk0500)\"", "ds_from_station": null, "organization_id": "a5877b42-96ea-4f13-af7e-246f355413d6", "doi_value": "", "subject_codes": [ "180.51" ], "quality_level": 1, "publish_time": "2024-12-09 16:46:30", "first_publish_time": "2024-12-09 16:46:30", "last_updated": "2025-12-09 19:20:21", "protected": false, "protected_to": "2025-10-31 00:00:00", "lang": "zh", "cstr": "33110.11.XIEG.xjsedata.2022.00009108", "license": null, "extra": null, "files_shape": [], "features": null, "data_level": 0, "i18n": { "en": { "title": "DNA Barcode Database of Key Species in Tianshan Wild Fruit Forest (2023)", "ds_abstract": "Plant DNA barcode sequences in Ili area, including matK, rbcL and ITS gene sequences and peak maps. 306 plant samples were sequenced and analyzed, belonging to 65 families, 204 genera, and 274 species. These include wild forest fruit plants, wild flowers, medicinal plants and crop relatives, rare and endangered plants, etc. MatK, rbcL and ITS sequencing showed that a total of 281 rbcL fragments, 281 matK fragments and 278 ITS fragments were obtained, each sequencing containing upstream primers (_f) and downstream primers (_r)
", "ds_source": "Sequencing raw data without processing.
", "ds_process_way": "Plant samples were collected in the wild, DNA extraction, PCR amplification, and base sequencing were obtained. Plant DNA bases are sequenced by a sequencer, the accuracy of base reading is judged based on the peak diagram, and the uncertain sequence at the second end of the sequence is removed to obtain a base sequence with high credibility. fasta sequencing results can be opened in text; peak map files (AB1 format) can be viewed using Chromas software.
", "ds_quality": "The collection process of DNA barcode data resources and genetic materials for key species in wild fruit forests in Tianshan Mountains is manually collected in accordance with the \"Technical Regulations on Investigation and Evaluation of Terrestrial Higher Plant Diversity in Counties\". The collection of DNA barcode data refers to and quotes the \"Technical Specifications for Research on Plant DNA Barcoding\"(Gao Lianming et al., 2012) and relevant content of the biodiversity survey specifications for this scientific expedition. After manual inspection, the error value of this year's data is less than 0.1%, which meets the relevant requirements of the biodiversity survey specifications for this scientific expedition.
", "ds_acq_place": "Yili area", "ds_ref_instruction": "The DNA barcode database of key species in Tianshan wild fruit forest (2023) comes from the third Xinjiang comprehensive scientific expedition special project \"Tianshan wild fruit forest ecosystem and biodiversity survey (2021 xjk0500)\"", "ds_format": "form", "ds_projection": "no" } }, "license_type": null, "doi_reg_from": "reg_local", "cstr_reg_from": "reg_local", "doi_not_reg_reason": null, "cstr_not_reg_reason": null, "is_paper_in_submitting": false, "ds_topic_tags": [ "植物", "DNA条形码", "DNA基因序列" ], "ds_subject_tags": [ "植物学" ], "ds_class_tags": [], "ds_locus_tags": [ "伊犁地区" ], "ds_time_tags": [ 2023 ], "ds_contributors": [ "杨红兰" ], "ds_meta_authors": [ "杨红兰" ], "ds_managers": [ "杨红兰" ], "category": "生物多样性" }